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KMID : 0380020090240020195
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 2 p.195 ~ p.200
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Improvement of Growth and Benzo[c]phenanthridine Alkaloids Production by Modifying Nitrogen Source in Suspension Cell Culture of Eschscholtzia californica
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Lee Song-Eun
Rhee Hong-Soon
Son Seok-Young
Park Jong-Moon
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Abstract
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The effect of nitrogen source on cell growth and benzo[c]phenanthridine alkaloids production by modifying ratio in cell suspension culture of Eschscholtzia califarnica was investigated. When total nitrogen concentration is maintained (60 mM), maximum benzo[c]phenanthridine alkaloids production is about 60.72 mg/L at 50:10 (mol/mol). This productivity was 3.8 times higher than that obtained when cells were grown instandard MS medium. The decrease of ratio at 60 mM of total nitrogen caused the decline of both growth and benzo[c]phenanthridine alkaloids production. Under the same concentration of (50 mM), higher concentration of inhibited cell growth strongly but induced alkaloids production slightly. Also, under the same concentration of (25 mM), higher concentration of induced alkaloids production strongly but high concentration of (100 mM) interfered alkaloids instead. Maximum benzo[c]phenanthridine alkaloids production is about 62.71 mg/L at 50:25 (mol/mol). These results suggest that higher biomass and higher alkaloids production could be obtained by optimizing each nitrogen concentration as well as ratio in the culture medium. Nitrate and ammonium in culture medium have distinct role in the regulation of growth and alkaloids production; ammonium had a strong influence on growth while nitrate had an influence on alkaloids production.
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KEYWORD
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Eschscholtzia califarnica, benzo[c]phenanthridine alkaloids, growth, nitrate, ammonium
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